Crystallization and preliminary X-ray diffraction studies of mandelonitrile lyase from almonds.
نویسندگان
چکیده
Single crystals of three different isoenzymes of (R)-(+) mandelonitrile lyase (hydroxynitrile lyase) from almonds (Prunus amygdalus) have been obtained by hanging drop vapor diffusion using polyethylene glycol 4000 and isopropanol as co-precipitants. The crystals belong to the monoclinic space group P2(1) with unit cell parameters a = 69.9, b = 95.1, c = 95.6 A, and beta = 118.5 degrees. A complete set of diffraction data has been collected to 2.6 A resolution on native crystals of isoenzyme III.
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ورودعنوان ژورنال:
- Proteins
دوره 19 4 شماره
صفحات -
تاریخ انتشار 1994